The results of tecnological research are rapresented by the identification of enzimatic activities and microorganism able to degrade contaminants in raw material and food. The identification has been performed by using both a classical microbiological approach and the analysis of Next Generation Sequencing data. Microorganism were selected based on their ability of survive and adapt to polluted environments. Growth assays and chemical and transcriptomic analysisin the presence of the pollutant were performed in order to identify the enzymes responsible for degradation. The candidate enzymes were purified and validated through in vitro degradation assays.
- coupled use of classical microbiological methods and high-throughput molecular biology technologies;
- Identification of enzymes capable of degrading more contaminants simultaneously;
- alternative use of edible fungi for biotechnological applications.