The TBP method, developed for the qualitative and quantitative identification of primary, raw material of both animal and plant origin that may be present in food, is unique, different from any other method used by the competition for the same purpose. Protected by patents and pending patents own by the Italian National Research Council, is capable of tracing any element, species or varieties, declared or not, actually present in food mixtures and primary raw material. It is based on a convenient and simple PCR reaction followed by the resolution of the amplified fragments done through capillary electrophoresis. A specific, distinct and discriminatory tubulin based barcode (TBB) is thus assigned to any analyzed specie or variety, to any raw material, to any mixture. TBB can be translated into a QR code that can be read with the use of any smart device. In addition, each of the multiple products of amplification can be used to produce species- or varieties-specific molecular probes useful for quantitative determinations. Because of its large versatility and applicability, the number of distinctive genomic profiles and molecular probes can increase at will, at costumer request.
At the moment, nothing is even close to the TBP-based method for the identification of food composition, varieties and species, or contaminants in primary raw materials. This is because the rationale that led to its development is completely different from those on which routine molecular biology techniques are based. This is the reason why the TBP-method releases a true DNA barcode conveying information about the biological composition of food matrices. Such a barcode can be translated in a convenient QR code delivering information about food content and composition and raw material purity or contamination. No restrictions of its applications to animal or plant derived products are met. In addition, it can be exploited for the preparation of custom-made molecular probes that can be used for quantitative determinations of single species and varieties. The TBP method can be easily implemented on demand, following market or customer needs. The cost of the analyses, already provided to some important companies working in the food sector with a higher interest in products of plant origin, are truly competitive.