The technology is based on the development of molecular markers suitable to the identification and quantification of the precious white truffle - Tuber magnatum Pico – in truffle-products in order to certify their quality to the consumer.
The identification of the truffle species is generally based on the features of their spores and peridium, but the identification in processed truffle-products as creams and butter can be ambiguous because of the drastic treatments to which truffles are exposed during the commercial preparation.
Furthermore, when immature truffles are used, the spores are not present and can not be a tool useful for the identification of the truffle species used in the preparation of the products that contain truffles among the ingredients.
Primers specific for Tuber magnatum have been developed and used in quantitative PCR (qRT‐PCR), allowing the quantification in real time of the white truffle in processed products.
The developed technique is so sensitive that detects small amount of target DNA (up 10‐5 ng/μL) and can be succesfully applied in easy way and at low cost for the detection of frauds due to the use of less precious truffle species such as the “ bianchetto” Tuber borchii, in truffle-products.
Given that the precious white truffle grows only in Italy and in a few areas in East Europe, in spite of other truffle species, but it is consumed in all the world, a method based on DNA useful for its unambiguous authentication in truffle-products is a powerful tool for the consumer who is willing to spend a lot for such a geographically limited product.